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KMID : 0613820090190070866
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Journal of Life Science
2009 Volume.19 No. 7 p.866 ~ p.870
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Regulation of the Drosophila Fascin by Raf Signaling
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Pyo Jung-Hoon
Choi Na-Hyun
Lee Shin-Hae
Kim Young-Shin
Yoo Mi-Ae
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Abstract
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Fascin, a cytoskeleton actin binding protein, functions in cell adhesion and cell migration. Fascin is also known as a candidate biomarker for various cancers, however, regulatory mechanisms of fascin expression remains little understood. In this study, we found an abnormal bristle phenotype, which is similar to that of the Drosophila fascin mutant, in Draf mutant flies. Hence, we investigated whether fascin expression is regulated by Raf signaling. RT-PCR and Western blot analysis showed that Drosophila fascin expression was down-regulated in Draf mutant flies and the level was increased in larvae expressing the oncogenic form of Draf (Draf gof) under the GAL4-UAS system. Immunostaining analysis showed increased fascin in the hemocytes over-expressing Draf gof. Our results indicate that fascin expression is regulated by Raf signaling and suggest that Raf signaling may play an important role in the actin cytoskeleton-associated developmental process and tumor progression via regulation of fascin gene.
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KEYWORD
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Drosophila, fascin, singed, Raf, gene expression
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